University of Benin, Nigeria
Title: BIO-ASSAY GUILDED ISOLATION AND CHARACTERIZATION OF HEPATOPROTECTIVE COMPOUNDS FROM Tetracarpidium conophorum SEEDS(AFRICAN WALNUT)
Biography: Dr. Kelly Oriakhi
Tetracarpidium conophorum is a tropical plant used in Nigerian ethno pharmacological system of medicine for treating various diseases including hepatic ailments. The present study was designed to investigate bioassay guided isolation of hepatoprotective compounds from T. conophorum seeds in carbon tetrachloride (CCl4) induced liver damage. Powdered seeds of T. conophorum were macerated in methanol for 72 hrs. The methanol extract was partitioned into n-hexane(HE), dichloromethane(DCM) and ethyl acetate (EA) fractions. The methanol extract and fractions were tested in-vivo for their hepatotoprotective activities in CCl4 rats model.The most active HE and EA fractions were subjected to column chromatography led to the isolation of nine known compounds respectively, Betulinic acid(1), β-sitosterol(2), sitosterol-3-O-[ β –D glucopyranoside](3), stimagsterol(4), ricinine(5),α-amyrin(6), gallic acid(7), keampferol(8), apigenin(9). All compounds were identified by UV, IR, MS and NMR spectroscopy.